ABSTRACT
Body exposure to fine particulate matter air pollution (PM2.5) can cause a variety of health damage. PM2.5 can act directly on the respiratory system and through the blood barrier, which is closely related to the body's various diseases. This paper elaborated possible mechanism of PM2.5 on the respiratory system as well as varying degrees of damage,pointed out the mechanism of toxicity, and a more in-depth toxicity mechanism from epidemiological studies. Meanwhile, this paper provides a scientific basis about PM2.5 and other cutting-edge issues.
ABSTRACT
Aim To develop a new method for easy endotrache-al intubation and instillation in mice. Methods Relying on the endoscope of cold light and gel loading tips, a new method was set up for intubated operations. Under high magnification, oper-ations of endotracheal intubation and instillation were completed in mice. Twenty male CB17-SCID mice ( between 24 and 28 g) were repeatedly intubated and instilled with 80 μL suspensions of air polluted fine particulate matters in a week. Results The twenty mice were sacrificed after 24h of the last instillation. The dispersions of the air polluted fine particulate matters were found at the bottoms of tracheas and in both the left and right lungs in all mice. Conclusion Using the endoscope of cold light and gel loading tips, a new method for tracheal instillation is set up in mice, which is rapid, simple and has good repeatability. It could be widely applied for the endotracheal intubation and in-stillation in mice.